Micrococcal Nuclease
货号:
10011S 基本售价:
778.0 元 规格:
60ul
产品信息
概述货号 | 10011S |
应用 | ChIP |
使用方法 | Use during chromatin fragmentation in chromatin immunoprecipitation (ChIP) assays as directed in the protocols for the SimpleChIP®and SimpleChIP® Plus Enzymatic Chromatin IP Kits. |
参考图片HeLa cells were formaldehyde-crosslinked and chromatin was prepared and digested as described in Section A of the SimpleChip® Kit protocol using Micrococcal Nuclease for digestion. DNA was purified as described in Section B. 10 μl were separated by electrophoresis on a 1% agarose gel (lane 2) and stained with ethidium bromide. Lane 2 shows that the majority of chromatin was digested to 1 to 5 nucleosomes (150 to 900 bp). |