MACSelect 4 Transfected Cell Select. Kit
货号：
130-091-988 基本售价：
询价 规格：
EA
产品信息
概述| 货号 | 130-091-988 |
| 描述 | The MACSelect 4 System uses the transiently expressed truncated human CD4 molecule as a surface marker to select transfected cells. It can be used in virtually all CD4- adherent or suspension cell lines and primary cells in combination with common transfection methods. |
性能| 供应商 | Miltenyi |
| 背景 | The MACSelect 4 – Transfected Cell Selection Kit contains the vectors pMACS 4-IRES.II and pMACS 4.1, a control vector, MACSelect 4 MicroBeads, CD4-FITC antibody for detection of cells before enrichment, MACSelect Control FITC antibody for detection of cells after enrichment, and a CD14-FITC antibody for analysis of the control experiment when doing a co-transfection. All components are also available separately. The pMACS 4-IRES.II vector enables the bicistronic expression of the gene of interest from a CMV promotor and the truncated CD4 molecule via an internal ribosome entry side (IRES). The pMACS 4.1 vector encodes the truncated CD4 marker and is recommended for co-transfection in combination with an expression vector encoding the gene of interest. Transfected cells are magnetically labeled with CD4 MicroBeads and subsequently separated from non-transfected cells. |
