| 货号 | MAB457-500 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB457-500；Detection antibody：BAF457; and protein: 457-6C-025 |
| 别名 | 6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Immunohistochemistry,CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 6Ckine, rhMIP-1 alpha, rhMIP-1 beta, recombinant mouse (rm) MIP-1 alpha, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rhTeck, or rmTeck. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6366 (Human); 18829 (Mouse); 298006 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse CCL21/6Ckine Ser24-Gly133 Accession # P84444 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | 6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid, highly basic, precursor protein with a 23 aa signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid protein with a 23 signal peptide that is cleaved to generate the 110 aa mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% aa sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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