Human MMP-9 MAb (Clone 36020) (500 UG)【科瑞奥博】

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Human MMP-9 MAb (Clone 36020) (500 UG)

货号： MAB936-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB936-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB936-500；Detection antibody：BAF911; and protein: 911-MP-010
别名	92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP-9; type V collagenase
全称	Matrix Metalloproteinase 9
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunoprecipitation,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human MMP-9 in Western blots. Does not react with the C-terminal truncated form (65 kDa) of recombinant human (rh) MMP-9. In Western blots, approximately 5% cross-reactivity with rhMMP-2 and no cross-reactivity with rhMMP-1 or rhMMP-3 is observed.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4318 (Human); 17395 (Mouse); 81687 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies. Authors: Ohlsson S, Ohlsson S, Soderberg D, Gunnarsson L, Pettersson A, Segelmark M, Hellmark T Clin Exp Immunol, 2014;176(3):363-72. Species: Human Sample Type: cell supernates Application: ELISA Capture In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia. Authors: Schneider P, Costa O, Legrand E Leuk. Res., 2010;34(1):24-31. Species: Human Sample Type: Whole Cells Application: Flow Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro. Authors: Zhao Z, McCloud B, Fleming R, Klempner MS Biochem. Biophys. Res. Commun., 2007;358(2):528-33. Species: Human Sample Type: Cell Lysates Application: WB Distinct synovial immunopathologic characteristics of juvenile-onset spondylarthritis and other forms of juvenile idiopathic arthritis. Authors: Kruithof E, Van den Bossche V, De Rycke L, Vandooren B, Joos R, Canete JD, Tak PP, Boots AM, Veys EM, Baeten D Arthritis Rheum., 2006;54(8):2594-604. Species: Human Sample Type: Whole Tissue Application: IHC Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery. Authors: Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C Laryngoscope, 2005;115(1):56-61. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Increased production of matrix metalloproteinases in Helicobacter pylori-associated human gastritis. Authors: Bergin PJ, Anders E, Sicheng W, Erik J, Jennie A, Hans L, Pierre M, Qiang PH, Marianne QJ Helicobacter, 2004;9(0):201. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Pro Matrix Metalloproteinase 9 (Pro-MMP-9) is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9, which can degrade collagens and elastin as well as several non-ECM molecules.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling APP Cleavage and Amyloid-beta Degradation Cancer Biomarkers Cell to Cell Adhesion Disassembly During EMT Collagen Modifying Enzymes Extracellular Matrix Remodeling and Cell Migration During EMT Gametes and Fertilization ICC / IHC Images: MMP-9 Inflammatory Mediators Mesenchymal Cells and EMT Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression Preeclampsia

参考图片

MMP‑9 in SW1353 Human Cell Line. MMP‑9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MMP‑9 in Human Lung. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.