| 货号 | MAB3771-500 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB3771-500；Detection antibody：BAF377; and protein: 377-HB-050 |
| 别名 | Neuregulin1 beta 1; Neuregulin-1 beta 1/NRG1 beta 1 |
| 反应种属 | Human |
| 应用 | ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects human NRG1-beta 1 in ELISAs and Western blots. Recognizes an epitope found in the EGF-like domain of NRG1‑ beta 1 (amino acids 179‑246). It also detects recombinant human SMDF (Sensory and Motor neuron-Derived Factor), but it does not recognize the EGF-like domain of human NRG1-alpha (amino acids 177‑241). The epitope is present within amino acids 213‑230 of human NRG1-beta 1. This segment is present in all NRG1 beta isoforms, GGF‑2 (glial growth factor), and SMDF, but it is absent from NRG1-alpha, NDF43, and NRG1-gamma. |
| 使用方法 | Western Blot: 2 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3084 (Human); 211323 (Mouse); 112400 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activation of the neuregulin/ErbB system during physiological ventricular remodeling in pregnancy. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human NRG1-beta 1/HRG1-beta 1 Ser2-Lys246 Accession # AAA58639 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Neuregulin-1, also known as Heregulin 1, functions as a ligand for the receptor tyrosine kinases, ErbB3 and ErbB4. It exists in several different mRNA splicing variants resulting in both transmembrane and secreted forms. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human NRG1‑ beta 1/HRG1‑ beta 1 by Western Blot. Western blot shows lysates of Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Human NRG1-beta 1/HRG1-beta 1 Monoclonal Antibody (Catalog # MAB3771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRG1‑ beta 1/HRG1‑ beta 1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
