PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair
货号：
7945S 基本售价：
5950.0 元 规格：
1Kit
产品信息
概述| 货号 | 7945S |
| 描述 | CSTs PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total α-Tubulin Sandwich ELISA Kit #7944. Capture and detection antibodies (100X stocks) and an HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The α-tubulin rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an α-tubulin mouse detection antibody and HRP-linked, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of α-tubulin. Microtubule stabilizing or destabilizing agents may significantly increase or decrease the signal, respectively. |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
性能| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | 4C |
| 参考文献 | Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
|
参考图片Figure 1. The relationship between the protein concentration of the lysate from A-431 cells and the absorbance at 450 nm using the PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair #7945 is shown. 图1： A-431细胞裂解的蛋白浓度与在450 nm吸光度的关系通过使用PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair #7945被显示。 |
