PathScan® Total GFP Sandwich ELISA Antibody Pair
货号：
7879S 基本售价：
5950.0 元 规格：
1Kit
产品信息
概述| 货号 | 7879S |
| 描述 | CSTs PathScan® Total GFP Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total GFP Sandwich ELISA Kit #7878. This antibody pair is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency and may not detect GFP fusion proteins. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The GFP rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a GFP mouse detection antibody and HRP-conjugated, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP. |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
性能| 供应商 | CST |
| 背景 | The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency. |
| 存放说明 | 4C |
| 参考文献 | 1 . Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.
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参考图片Figure 1. The relationship between the protein concentration of the lysate from untransfected and GFP transfected HEK 293 cells and the absorbance at 450 nm using the PathScan® Total GFP Sandwich ELISA Antibody Pair #7879 is shown.图中所示为用 PathScan ® Total GFP Sandwich ELISA Antibody Pair #7879所做的未转染组和GFP转染后的HEK293细胞提取物中蛋白浓度与450nm处吸光值之间的相关性分析。 |
