| 货号 | 7824S |
| 描述 | CSTs PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit #7335. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The VEGFR-2 capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-VEGFR-2 (Tyr1175) detection antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-VEGFR-2 (Tyr1175) protein. |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). |
| 存放说明 | 4C |
| 参考文献 | Meyer, M. et al. (1999) EMBO J 18, 363-74. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7. Takahashi, T. et al. (2001) EMBO J 20, 2768-78. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4. |
The relationship between the protein concentration of lysate from HUVE cells, untreated or treated with VEGF, and the absorbance at 450 nM using PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Antibody Pair #7824 is shown. HUVE cells (85% confluence) were treated with VEGF (50 ng/ml) for 5 min. at 37ºC, and then lysed.用PathScan® Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Antibody Pair #7824检测得到的经过或未经VEGF刺激的HUVE细胞的裂解液中蛋白含量和450nm吸光值之间的关系。用VEGF (50 ng/ml)在37°C 刺激HUVE细胞（铺至85%满）5分钟，然后裂解细胞。 |
