PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair
货号：
7963S 基本售价：
5950.0 元 规格：
1Kit
产品信息
概述| 货号 | 7963S |
| 反应种属 | Human/Dog |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
性能| 供应商 | CST |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | 4C |
| 参考文献 | Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
Hunter, T. (1987) Cell 49, 1-4.
|
参考图片The relationship between the protein concentration of the lysate from untreated and H202-treated MDCK cells and the absorbance at 450 nm using the PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair #7963 is shown. MDCK cells were treated with H202 (3 mM) for 3 minutes at 37ºC and then lysed.图中显示了未经处理和H202处理的MDCK细胞溶解产物蛋白质浓度与450 nm 处光学密度读数之间的关系，使用的抗体是PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair #7963。MDCK细胞用H202 (3 mM)处理，37ºC 处理3min后裂解。 |
