| 货号 | 7343S |
| 描述 | CSTs PathScan® Phospho-IκB-α (Ser32) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Kit #7355. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IκBα Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-IκBα (Ser32) Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-IκBα (Ser32) protein. *Antibodies in this kit are custom formulations specific to the kit. |
| 反应种属 | Human/Mouse |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | 4C |
| 参考文献 | Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. Brown, K. et al. (1995) Science 267, 1485-8. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Chen, Z.J. et al. (1996) Cell 84, 853-62. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63. |
The relationship between lysate protein concentration from untreated and TNF-α and IL-1β treated HeLa cells and the absorbance at 450 nm using PathScan® Phopho-IκBα (Ser32) Sandwich ELISA Antibody Pair #7343 is shown. HeLa cells were treated with TNF-α and IL-1β for 5 minutes at 37ºC and then lysed.图示为未经处理和经TNF-α 和 IL-1β 处理的HeLa细胞的裂解液蛋白浓度与450 nm 吸收峰的关系，所用抗体为PathScan® Phopho-IκBα (Ser32) Sandwich ELISA Antibody Pair #7343 。在37ºC条件下，用TNF-α和IL-1β 处理HeLa细胞5min，然后裂解。 |
