| 货号 | 7828S |
| 描述 | CSTs PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Kit #7258. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The insulin receptor β capture antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by a phospho-insulin receptor β (Tyr1150/1151) detection antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-insulin receptor β (Tyr1150/1151) protein. |
| 反应种属 | Human/Mouse |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | 4C |
| 参考文献 | Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93. Baserga, R. (2000) Oncogene 19, 5574-81. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60. Baserga, R. (1999) Exp Cell Res 253, 1-6. White, M.F. et al. (1985) J Biol Chem 260, 9470-8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80. |
The relationship between protein concentration of lysates from untreated or insulin-treated CHO-IR/IRS-1 cells and the absorbance at 450 nm using PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Antibody Pair #7828 is shown. CHO-IR/IRS-1 cells (85% confluence) were serum starved overnight and then treated with insulin (100 nM) for 2 min at 37ºC, and then lysed. CHO-IR/IRS-1细胞裂解液蛋白浓度与450nm处的吸光度关系图，细胞未处理或经胰岛素处理，所用抗体为PathScan® Phospho-Insulin Receptor β (Tyr1150/1151) Sandwich ELISA Antibody Pair。处理方法如下：细胞（85%）饥饿后，用100 nM胰岛素于37度下处理2分钟，然后进行裂解。 |
