| 货号 | 7294S |
| 描述 | CSTs PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Kit #7231. Capture and Detection Antibodies (100X stocks) and HRP-Conjugated Streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The c-Kit Mouse Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Biotinylated Phospho-Tyrosine Mouse Detection Antibody and HRP-conjugated streptavidin. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-c-Kit (panTyr) protein. Antibodies in kit are custom formulations specific to kit. |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7). |
| 存放说明 | 4C |
| 参考文献 | Martin, F.H. et al. (1990) Cell 63, 203-11. Yarden, Y. et al. (1987) EMBO J 6, 3341-51. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53. Nocka, K. et al. (1990) EMBO J 9, 1805-13. Hirota, S. et al. (1998) Science 279, 577-80. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62. |
The relationship between protein concentration of lysates from untreated and SCF-treated H526 lysates and the absorbance at 450 nm using PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Antibody Pair #7294 is shown. Cells (0.5x106 cells/ml) were serum starved overnight and then treated with Human Stem Cell Factor (SCF) #9907 (40 ng/ml) for 5 min at 37°C.用PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Antibody Pair #7294检测得到的经过或未经SCF刺激的H526细胞的裂解液中蛋白含量和450nm吸光值之间的关系。0.5x106 cells/ml 的细胞血清饥饿过夜，然后用40 ng/ml SCF#9907在37°C.刺激5分钟。 |
