| 货号 | 7141S |
| 反应种属 | Human/Mouse/Rat |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12). |
| 存放说明 | 4C |
| 参考文献 | Jochum, W. et al. (2001) Oncogene 20, 2401-12. Davis, R.J. (2000) Cell 103, 239-52. Hilberg, F. et al. (1993) Nature 365, 179-81. Raivich, G. et al. (2004) Neuron 43, 57-67. Behrens, A. et al. (2002) EMBO J 21, 1782-90. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4. |
The relationship between lysate protein concentration from untreated and UV treated 293 cells and the absorbance at 450 nm using PathScan® Phospho-c-Jun (Ser 63) Sandwich ELISA Antibody Pair #7141 is shown. 293 cells were UV-treated, allowed to recover for 30 minutes at 37ºC and then lysed.图中显示了未经处理和紫外照射的293细胞溶解产物蛋白质浓度之间的关系，以及PathScan® Phospho-c-Jun (Ser 63) Sandwich ELISA Antibody Pair #7141抗体分析的450 nm 处光学密度读数。293细胞用紫外照射处理，37ºC 恢复30min后裂解。 |
