| 货号 | MAB3341 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB3341；Detection antibody：BAF334; and protein: 334-TK-025 |
| 别名 | C-C motif chemokine 25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects human CCL25/TECK in direct ELISAs and Western blots. Shows less than 1% cross-reactivity with recombinant mouse CCL25 and recombinant rat CINC-1 and no cross-reactivity with recombinant human (rh)CCL28, recombinant mouse (rm)CCL28, rhMPIF-1, rh6Ckine, rm6Ckine, rhEotaxin, or rmEotaxin. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6370 (Human); 20300 (Mouse) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL25/TECK Gln24-Leu150 Accession # AAB69981 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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