| 货号 | MAB508 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB508；Detection antibody：BAF508; and protein: 508-CK-025 |
| 别名 | C6 beta-chemokine; chemokine (C-C motif) ligand 23; Ck beta 8-1; CKb8; Ckb-8; CK-beta-8; hmrp-2a; Macrophage inflammatory protein 3; MIP3; MIP-3CK-BETA-8; MPIF1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; SCYA23C-C motif chemokine 23; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23 |
| 反应种属 | Human |
| 应用 | Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects human CCL23/Ck beta 8‑1 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with human CCL23/CK beta 8 isoform is observed. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6368 (Human) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL23/CK beta 8-1 Met46-Asn137 Accession # AAD10846 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL23 (SCY A23), a splice variant of CK beta 8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta 8-1, CK beta 8/MPIF-1 lacks a 17 aa residue stretch (Leu47‑Gly63) present in CCL23 and has a unique arginine at residue 46. CK beta 8-1/CK beta 8 (MPIF-1) and leukotactin/MIP-1δ form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1δ), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta 8-1 and CK beta 8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta 8 (MPIF-1), the truncated form of CK beta 8-1 (aa 46‑132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta 8-1 (aa 22‑132). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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