Purified Sp1 protein
货号：
31137 基本售价：
7177.5 元 规格：
2 ug
产品信息
概述| 货号 | 31137 |
| 使用方法 | Purified Sp1 is suitable for in vitro transcription, DNase I protection and gelshift assays. 50-100 ng is sufficient for reconstituted transcription assays and 5-25 ng in a 20 µl volume are sufficient for gelshift assays. The molecular weight of the protein is ~105 kDa. This protein is not recommended for TransAM®assays.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. |
性能| 供应商 | Active Motif |
| 背景 | Sp1(specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. |
| 运输条件 | Dry Ice |
| 外观 | 2 µg of Purified Sp1 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.08 µg/µl in Dilution Buffer AM1. |
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