Recombinant p300 protein
货号：
31124 基本售价：
7177.5 元 规格：
4 ug
产品信息
概述| 货号 | 31124 |
| 别名 | EP300, KAT3B, RSTS2 |
| 使用方法 | Recombinant p300 is suitable for protein-protein interaction, in vitrotranscription,in vitro acetylation and cell growth assays. 1-5 ng is sufficient for gelshift assays in a 20 µl volume, 50-100 ng is sufficient for reconstituted transcription assays and 100-200 ng is sufficient for protein-protein interaction or acetylation assays. The molecular weight of the protein is 265 kDa.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. |
性能| 供应商 | Active Motif |
| 背景 | E1A binding protein p300 (EP300)orp300 regulates cellular growth and differentiation and is also important in preventing tumor growth. It binds to transcription factors and functions as a coactivator of transcription. The p300 domain structure that facilitates interaction with transcription factors includes the bromodomain, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (TAZ1and TAZ2) and the interferon response binding domain (IBiD). Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for nonhistone targets. Specifically, p300 acetylates Lys131 of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TATs transactivating activity, and may aid induction of chromatin remodeling of proviral genes. |
| 运输条件 | Dry Ice |
| 外观 | 4 µg of Recombinant p300 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.2 µg/µl in Dilution Buffer AM1. |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
