Recombinant BRCA1 protein
货号：
31113 基本售价：
7177.5 元 规格：
2 ug
产品信息
概述| 货号 | 31113 |
| 别名 | IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53 |
| 使用方法 | Recombinant BRCA1 is suitable for in vitro function studies including transcription and DNA repair, for protein-protein interaction assays and cell growth assays. 1 ng is sufficient for a gelshift assay in a 20 µl reaction volume; 50 ng is sufficient for reconstituted transcription assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~205 kDa.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. |
性能| 供应商 | Active Motif |
| 背景 | Breast cancer type 1 susceptibility (BRCA1) protein is a tumor suppressor that is expressed in breast tissue cells and other tissues where it primarily functions to sense and repair DNA damage. BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of Lys6-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. BRCA1 associates with other tumor suppressors, DNA repair proteins, and signal transducers in a large complex known as the BRCA1-associated genome surveillance complex (BASC) that functions to identify double-strand DNA breaks and either repair them or destroy cells where DNA damage cannot be repaired. BRCA1 also associates with RNA pol II and histone deacetylase complexes to modulate transcription. In addition to its role in ubiquitination, DNA damage repair and transcriptional regulation, it is known to be required for normal cell cycle progression. Defects in BRCA1 are a cause of susceptibility to familial breast-ovarian cancer type 1 (BROVCA1), a condition associated with familial predisposition to cancer of the breast and ovaries, and pancreatic cancer type 4 (PNCA4). |
| 运输条件 | Dry Ice |
| 外观 | 2 µg of Recombinant BRCA1 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.4 µg/µl in Dilution Buffer AM1. |
| 参考文献 | |
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