Recombinant Mononucleosomes (H2A.X) - biotinylated
货号：
31582 基本售价：
6090.0 元 规格：
20 ug
产品信息
概述| 货号 | 31582 |
| 使用方法 | Recombinant Mononucleosomes (H2A.X) - biotinylated, are suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
性能| 供应商 | Active Motif |
| 背景 | In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp ofDNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subjectto posttranslational modifications, such as methylation, acetylation, phosphorylation, mono-ubiquitination, etc.Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the “major“ histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple biology processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing.
H2A.X (also known as H2AFX, Histone Family Member X) is a histone H2A family member. In mammals, variant histone H2A.X represents 10% of the total H2A, whereas in yeast 90% of H2A is of the H2A.X class. The main function of H2AX is associated with the DNA damage repair (DDR) system which is induced by DNA double strand breaks (DSBs). In many species, including S. cerevisiae, Xenopus laevis, and Drosophila melanogaster, the fourth serine residue from the carboxyl-terminal end of histone H2A becomes phosphorylated. In mammals, this event is related to phosphorylation of the H2A.X variant histone on serine 139 by DNA-dependent protein kinases (ATR/ATM/DNA- PK). The phosphorylated form of H2A.X is commonly denoted as γ-H2A.X. This H2A.XS139 phosphorylation facilitates the recruitment of the DNA damage repair machinery, as well as chromatin remodelers such as INO80 and SWR1. H2A.X is a regulator of the epithelial–mesenchymal transition.
Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant Mononucleosomes (H2A.X) - biotinylated (20 µg protein + 19.2 µg DNA) are supplied in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration. |
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