| 货号 | 31426 |
| 别名 | Lysine Demethylase 1A, Flavin-Containing Amine Oxidase Domain-Containing Protein 2, Lysine-Specific Histone Demethylase 1 |
| 使用方法 | Recombinant LSD1 / KDM1A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me2 and H3K4me1 demethylase. Catalytic Ability: > 600 turnovers / enzyme molecule. Histone Demethylase Assay Conditions: 3.3 μM H3K4me2 (1-21aa) peptide was incubated with 5 nM KDM1A / LSD1 in reaction buffer containing 50 mM TrisCl, pH 8.0, 0.02% Triton X-100, 50 nM FAD for 1 hours at room temperature. MALDI-TOF were used for detection. |
| 供应商 | Active Motif |
| 背景 | LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant KDM1A / LSD1 protein was expressed in Sf9 cells at a concentration of 0.4 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
| 参考文献 | This protein is described in the following references: Lan F et al.(2007)Nature448:718-722 Forneris F et al.(2007)J Biol Chem.281:35289-35295 |
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Recombinant LSD1 / KDM1A protein gel. LSD1 / KDM1A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant LSD1 / KDM1A activity assay. 3.3 μM H3K4me2 (1-27aa) peptide was incubated with KDM1A / LSD1 in reaction buffer for 1 hour at room temperature. KDM1A / LSD1 enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K4me0) was measured using H3K4me0-specific antibody. | |
Recombinant LSD1 / KDM1A activity assay. 3.3 uM H3K4me2 peptide was incubated with 5 nM LSD1 in reaction buffer for 1 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me2 peptide was used as negative control. Catalytic Ability: >600 turnovers / enzyme molecule. |
