| 货号 | 31373 |
| 别名 | CBP; RSTS; KAT3A |
| 使用方法 | Recombinant CREBBP (1081-1197) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | CREB-binding protein (CREBBP)orCBP, is a protein known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. The CREBBP bromodomain recognizes acetylated histone lysine residues and functions as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. CREBBP has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. CREBBP acetylates histones as well as non-histone proteins, like NCOA3 coactivator. In addition, CREBBP binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. CREBBP also has been shown to act as a coactivator of ALX1 in the presence of p300. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant CREBBP (1081-1197) protein expressed in E. coli at a concentration of 4 mg/ml in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. |
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Recombinant CREBBP (1081-1197) protein gel. CREBBP (1081-1197) protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant CREBBP (1081-1197) activity using AlphaScreen. CREBBP (1081-1197) titration and inhibition were assessed using an AlphaScreen® assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. An IC50 dose response assessment of reference compound JQ1 is also shown. This data was generated and kindly provided courtesy of ChemPartner. |
