Recombinant METTL3 protein
货号：
31567 基本售价：
5872.5 元 规格：
20 ug
产品信息
概述| 货号 | 31567 |
| 使用方法 | Recombinant METTL3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Activity Assay Conditions: 1 µM substrate ssRNA oligos (5’-GUUGCCUGUUCGUGUUGGACUUGCCUGU-3’) and 1 µM SAM were incubated with METTL3 protein in EPI diluent buffer provided by Cisbio at room temperature for 1 hour (0.2 U/µL RNase inhibitor was added in this system). SAH-d2 and anti-SAH-K were added to the products, then HTRF signals were read after another 1 hour incubation. EPIgeneous™ Methyltransferase Assay kit from Cisbio was used for activity assay. |
性能| 供应商 | Active Motif |
| 背景 | N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs).
METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant METTL3 protein is supplied at a concentration of 0.35 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
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