Recombinant FTO protein
货号：
31572 基本售价：
5872.5 元 规格：
20 ug
产品信息
概述| 货号 | 31572 |
| 使用方法 | Recombinant FTO protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
3 µM m6A ssDNA oligos (DNA sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3’) were incubated with FTO protein in reaction buffer including 50 mM HEPES pH 7.5, 100 µM 2OG, 100 µM Ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Dot-Blot was used for activity detection. |
性能| 供应商 | Active Motif |
| 背景 | FTO (Alpha-Ketoglutarate Dependent Dioxygenase, also known as GDFD, ALKBH9, BMIQ14) is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily. FTO specifically demethylates m6A RNA. FTO plays an important role in the regulation of the global metabolic rate, energy expenditure and energy homeostasis. It is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant FTO protein is supplied at a concentration of 0.25 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
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