Recombinant AGO2 protein
货号：
31486 基本售价：
5872.5 元 规格：
20 ug
产品信息
概述| 货号 | 31486 |
| 别名 | Q10; EIF2C2 |
| 使用方法 | Recombinant AGO2 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling.Specific Activity: RNA endonuclease activity. |
性能| 供应商 | Active Motif |
| 背景 | AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10; EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleave the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3’ untranslated region (3’UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant AGO2 protein expressed in Sf9 cells at a concentration of 0.4 µg/µl in 25 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3X FLAG peptide. |
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参考图片Recombinant AGO2 protein gel.
AGO2 protein was run on an SDS-PAGE gel and stained with Coomassie blue. |
