| 货号 | 31193 |
| 别名 | RPS6KB1, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha |
| 使用方法 | Recombinant S6K is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.253 kDa. The activity of the protein is ~ 13 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK), 5 µg / 50 µl, Recombinant S6K: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 运输条件 | Dry Ice |
| 外观 | 10 µg of Recombinant S6K protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.422 µg/µl. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant S6K protein tested by SDS-PAGE gel. 0.5 µg of S6K protein was analyzed using SDS/PAGE followed by Western blot with either an anti-S6K or anti-GST antibody. |
