货号 | 31175 |
别名 | IGFR, CD221, IGFIR, JTK13 |
使用方法 | Recombinant IGF1-R is suitable for kinase assays and Western blot. The molecular weight of the protein is ~76.532 kDa. The activity of the protein is ~ 134 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 2 µg/50 µl, Recombinant IGF1-R: 20 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
供应商 | Active Motif |
运输条件 | Dry Ice |
外观 | 10 µg of Recombinant IGF1-R protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.09 µg/µl. |
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Recombinant IGF1-R protein tested by SDS-PAGE gel. 0.5 µg of IGF1-R protein was analyzed using SDS/PAGE followed by Western blot with either an anti-IGF11-R or anti-GST antibody. |