| 货号 | 55012 |
| 使用方法 | The β-Glucosyltransferase enzyme can be used to glucosylate 5-hmC DNA. Glucosylated DNA can be used as a differentiator with glucosyl-sensitive restriction enzymes. The use of a labeled [14C] UDP-Glucose donor would enable labeling of the 5-hmC residues. |
| 供应商 | Active Motif |
| 运输条件 | Blue Ice |
| 存放说明 | Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 200,000 Units β-Glucosyltransferase enzyme 10X β-Glucosyltransferase Reaction Buffer 50 mM UDP-Glucose 1 M DTT |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
β-Glucosyltransferase enzyme protection of 5-hmC DNA shown by agarose gel. 0.5 µg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated in the absence or presence of 1 unit β-Glucosyltransferase enzyme for 1 hour at 37°C. Following column purification, reactions were treated with 4 units of glucosyl-sensitive restriction enzyme GlaI and run on a 2.5% agarose gel. |
