Recombinant HDAC11 (2-347) protein
货号：
31538 基本售价：
5872.5 元 规格：
20 ug
产品信息
概述| 货号 | 31538 |
| 别名 | HDAC-11, Histone Deacetylase 11, HD11 |
| 使用方法 | Recombinant HDAC11 protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
HDAC Activity Assay Conditions: 1 µM H3K9ac peptide was incubated with different concentrations of HDAC11 protein in a 10 µl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl and 0.1 mg/ml BSA for 30 minutes. 10 µl H3K9ac0 antibody and SA-CL665 mixture (each at 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All reactions were performed at room temperature. HTRF assay was used for detection. |
性能| 供应商 | Active Motif |
| 背景 | HDAC11 (Histone Deacetylase 11) is a member of the class IV mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.
In humans, expression of HDAC11 is limited to only a few organs, including brain, heart, muscle and kidney, suggesting an important role of HDAC11 in those tissues. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant HDAC11 (2-347) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration. |
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