Recombinant AGO1 protein
货号：
31522 基本售价：
5872.5 元 规格：
20 ug
产品信息
概述| 货号 | 31522 |
| 别名 | Argonaute 1, Argonaute1, RISC Catalytic Component, EIF-2C, EIF2C, EIF2C1, GERP95, Q99 |
| 使用方法 | Recombinant AGO1 is suitable for use in the study of miRNA screening, RNAi research and selectivity profling. |
性能| 供应商 | Active Motif |
| 背景 | AGO1 (argonaute RISC catalytic component 1, also known as Q99; EIF2C1) is a member of the Argonaute family of proteins which play a role in RNA interference. The Argonaute family proteins (AGO1, AGO2, AGO3 and AGO4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleaves the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3’ untranslated region (3’-UTR). AGO1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant full length AGO1 protein was expressed in Sf9 cells and is provided in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mg/ml 3X Flag peptide. Please refer to product insert upon arrival for lot-specific concentration. |
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