BrdU Cell Proliferation Assay Kit
货号：
K306-1000 基本售价：
9620.0 元 规格：
1000assays
产品信息
概述| 货号 | K306-1000 |
| 描述 | 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Biovisions BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Applications - Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.• Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and |
| 样本类型以及上样量 | • Adherent and suspension cells |
| 产品特色 | • Convenient & non-radioactive• High-throughput suitable• Highly-sensitive: detect as low as 50-100 proliferating cells. • Suitable for cell proliferation, chemotaxis and cytotoxicity |
| 试剂盒组分 | • BrdU (1000X)• Fixing/Denaturing Solution• BrdU Detection Antibody (300X)• Anti-mouse HRP-linked Antibody (2000X)• Antibody Diluent• Wash Buffer (10X)• TMB Substrate• Stop Solution |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
