Quick Cell Proliferation Colorimetric Assay Kit
货号:
K301-2500 基本售价:
5826.5 元 规格:
2500assays
产品信息
概述货号 | K301-2500 |
描述 | The Quick Cell Proliferation Assay Kit provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in a microtiter plate. |
克隆号 | BV31-9 |
同种亚型 | Mouse IgG1 |
来源宿主 | Mouse |
性能供应商 | Biovision |
产品特色 | • Simple one-step procedure; takes less than 4 hours • Fast and convenient • The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate. |
试剂盒组分 | • WST-1 Reagent (lyophilized) • Electro Coupling Solution (ECS) |
运输条件 | Blue Ice |
存放说明 | -20℃ |