Glutathione Fluorometric Assay Kit
货号：
K251-100 基本售价：
4689.5 元 规格：
100assays
产品信息
概述| 货号 | K251-100 |
| 描述 | Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. BioVision’s ApoGSH™ Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 380/461 nm) • Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The Glutathione Detection Kit pro |
| 产品特色 | • Simple two-step procedure; takes only 2 hours • Fast and convenient • The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue when bound to glutathione. |
| 试剂盒组分 | • Cell Lysis Buffer • Monochlorobimane• GST Reagent• GSH Standard (1 mg; MW: 307) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
