Mitochondrial Apoptosis Detection Fluorometric Kit, MitoCapture
货号：
K250-25 基本售价：
2172.0 元 规格：
25assays
产品信息
概述| 货号 | K250-25 |
| 描述 | Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm). |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence microscopy and Flow cytometry: FITC (Ex/Em = 488/530 ± 30 nm); and (optional) PI (Em = 488/590 ± 42 nm) • Sample type- Live cells • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and100 assa |
| 产品特色 | • Simple one-step procedure; takes only 30 minutes • Fast and convenient |
| 试剂盒组分 | • MitoCapture Reagent • Incubation Buffer |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
