ApoBrdU DNA Fragmentation Assay Kit
货号：
K401-60 基本售价：
7641.5 元 规格：
60assays
产品信息
概述| 货号 | K401-60 |
| 描述 | Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody. |
| 同种亚型 | rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Sample type- Cell culture (adherent and suspension), parafin embedded tissue sections • Species reactivity- Mammal |
| 产品特色 | • Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods. |
| 试剂盒组分 | • Positive Control Cells • Negative Control Cells • Wash Buffer • Reaction Buffer • TdT Enzymes • Br-dUTP • Rinse Buffer • Anti-BrdU-FITC Antibody • PI/RNase Staining Buffer |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
