ApoDIRECT DNA Fragmentation Assay Kit
货号:
K402-50 基本售价:
7399.5 元 规格:
50assays
产品信息
概述货号 | K402-50 |
描述 | Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s ApoDIRECT In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy or analyzed by flow cytometry. |
同种亚型 | Rabbit IgG |
来源宿主 | Rabbit |
性能供应商 | Biovision |
检测类型 | Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters) • Sample type- Cell culture (adherent and suspension) • Species reactivity- Mammalian • Applications- The TU |
产品特色 | • Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The assay offers an one-step labeling of apoptotic cells. |
试剂盒组分 | • Positive Control Cells • Negative Control Cells • Wash Buffer • Reaction Buffer • TdT Enzymes • FITC-dUTP • Rinse Buffer • PI/RNase Staining Buffer |
运输条件 | Blue Ice |
存放说明 | -20℃ |