DNA Damage Quantification Colorimetric Kit
货号:
K253-25 基本售价:
5947.5 元 规格:
25assays
产品信息
概述货号 | K253-25 |
描述 | Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed duringthe course of base excision and repair of oxidized, deaminated or alkylated bases. It hasbeen estimated that about 2x105 base lesions are generated per cell per day. The level ofAP sites in cells can be a good indicator of DNA lesion and repair against chemicaldamage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (AldehydeReactive Probe) reagent that reacts specifically with an aldehyde group which is the openring form of the AP sites. After treating DNA containing AP sites with ARP reagents, APsites are tagged with biotin residues, which can be quantified using avidin-biotin assayfollowed by a colorimetric detection. The kit provides the necessary reagents forconvenient determination of abasic sites in purified DNA sample in 96-well plate format. |
同种亚型 | Rabbit IgG |
来源宿主 | Rabbit |
性能供应商 | Biovision |
检测类型 | Absorbance (450 and 650 nm)• Species reactivity- Mammalian • Applications- Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA |
样本类型以及上样量 | Purified damaged DNA |
产品特色 | Kit provides all necessary reagents for 96-well plate format |
试剂盒组分 | • ARP Solution • TE Buffer • Glycogen Solution • 0 ARP-DNA Standard• 40 ARP-DNA Standard• DNA Binding Solution • HRP-Streptavidin• 10X Wash Buffer • HRP Developer • 96-well Microplate (8 x 12 strips) |
运输条件 | Blue Ice |
存放说明 | -20℃ |