Caspase-8 Fluorometric Assay Kit
货号：
K112-400 基本售价：
10220.0 元 规格：
400assays
产品信息
概述| 货号 | K112-400 |
| 描述 | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by FLICE or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (400 nm excitation filter and 505 nm emission filter)• Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/midd |
| 产品特色 | • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity. |
| 试剂盒组分 | • Cell Lysis Buffer • 2X Reaction Buffer • IETD-AFC (1mM) • DTT (1 M) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
