Caspase-3 Colorimetric Assay Kit
货号：
K106-400 基本售价：
10220.0 元 规格：
400assays
产品信息
概述| 货号 | K106-400 |
| 描述 | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (400 or 405 nm) • Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/middle stages of apoptosis; differentiate |
| 产品特色 | • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. |
| 试剂盒组分 | • Cell Lysis buffer • 2X Reaction Buffer • DEVD-pNA (4 mM) • DTT (1 M) • Dilution Buffer |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
