CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)
货号：
K163-100 基本售价：
7762.5 元 规格：
100assays
产品信息
概述| 货号 | K163-100 |
| 描述 | Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 370-425/ 490-525 nm) • Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective immunosorbent enzyme assay for specific, quantitative detectio |
| 产品特色 | • Simple one-step procedure; takes only ~3 hours • Fast and convenient |
| 试剂盒组分 | • Cell Lysis Buffer • Coating Buffer • Anti-Caspase-3 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1 M) • DEVD-AFC Substrate (1 mM) • Positive Control (rh-Caspase-3) • Microtiter Plate • Adhesive Plate Cover |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
