| 货号 | K169-100 |
| 描述 | Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In BioVision’s Granzyme B Inhibitor Screening Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 试剂盒组分 | • Granzyme B Assay Buffer • Granzyme B Substrate • Granzyme B Enzyme (human recombinant) • Inhibitor Control (Ac-IEPD-CHO, 250 μM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |