Caspase-3 Inhibitor Drug Screening Kit (Fluorometric)
货号：
K153-100 基本售价：
5584.5 元 规格：
100assays
产品信息
概述| 货号 | K153-100 |
| 描述 | Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-3 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-3 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 400/505) • Sample type- Cell samples treated with Caspase inhibitor drugs • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective means for screening caspase inhibitors using fluoro |
| 产品特色 | • Simple one-step procedure; takes only 1-1.5 hours • Fast and convenient • Inhibition of caspase-3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. |
| 试剂盒组分 | • 2X Reaction Buffer • Caspase Substrate DEVD-AFC (1 mM) • DTT (1 M) • Active Caspase-3 (Lyophilized) • Caspase-3 Inhibitor |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
