Annexin V-FITC Early Apoptosis Detection Kit【科瑞奥博】

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Annexin V-FITC Early Apoptosis Detection Kit

货号： 6592S 基本售价： 5458.0 元规格： 1Kit

产品信息

概述
货号	6592S
描述	The Annexin V-FITC Early Apoptosis Detection Kit enables researchers to identify early apoptotic cells within a cell population. Annexin V-FITC conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Cells stained with propidium iodide (PI), a non-cell-permeable DNA dye, indicate necrotic cells. Cells stained with both PI and annexin V-FITC demonstrate later stage apoptosis and early necrosis. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume.
反应种属	All
应用	IF-IC/F

性能
供应商	CST
背景	Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same charactersistic of Ca2+-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).
存放说明	4C
参考文献	1 . Boersma, H.H. et al. (2005) J Nucl Med 46, 2035-50. 2 . Vermes, I. et al. (1995) J Immunol Methods 184, 39-51.

参考图片

Confocal immunofluorescent analysis of live Jurkat cells, untreated (left) or camptothecin-treated (right), using Annexin V-FITC Conjugate (green). Red = Propidium Iodide (fluorescent DNA dye). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析活的Jurkat细胞，未处理（左图）或用喜树碱处理（右图），使用的抗体是：Annexin V-FITC结合物（绿色）。红色=碘化丙啶（DNA荧光染料）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Flow cytometric analysis of Jurkat cells untreated (left) or treated with Staurosporine #9953 (1 μM, 2 hr; right) using Annexin V-FITC Early Apoptosis Detection Kit.流式细胞检测分析Jurkat细胞，未处理（左图）或用Staurosporine #9953 (1 μM, 2小时; 右图)处理，使用Annexin V-FITC Early Apoptosis Detection Kit。