Cathepsin S Activity Fluorometric Assay Kit
货号：
K144-100 基本售价：
4979.5 元 规格：
100assays
产品信息
概述| 货号 | K144-100 |
| 描述 | Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-S Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate Z-VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 400/505) • Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that |
| 产品特色 | • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Cathepsin-S assay is straightforward, and can be adapted to 96-well plate assays. |
| 试剂盒组分 | • CS Cell Lysis Buffer • CS Reaction Buffer • CS Substrate Z-VVR-AFC (10 mM)• CS Inhibitor (1 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
