Caspase-12 Fluorometric Assay Kit
货号：
K139-100 基本售价：
4858.5 元 规格：
100assays
产品信息
概述| 货号 | K139-100 |
| 描述 | Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 400/505)• Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100 assays) • Applications- Detect early/middle stages of apoptosis; differentiate apoptosis |
| 产品特色 | • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity. |
| 试剂盒组分 | • Cell Lysis Buffer • 2X Reaction Buffer • ATAD-AFC Substrate • DTT (1 M) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
