Caspase-3 Fluorometric Assay Kit
货号：
K105-100 基本售价：
4689.5 元 规格：
100assays
产品信息
概述| 货号 | K105-100 |
| 描述 | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity. |
| 克隆号 | HCP-B2 |
| 同种亚型 | Mouse IgG1 |
| 来源宿主 | Mouse |
性能| 供应商 | Biovision |
| 检测类型 | fluorecence (400 nm excitation filter and 505 nm emission filter)• Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/middl |
| 产品特色 | • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity |
| 试剂盒组分 | • Cell Lysis Buffer • 2X Reaction Buffer • DEVD-AFC (1 mM) • DTT (1 M) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
