Caspase-1 Fluorometric Assay Kit
货号：
K110-25 基本售价：
2172.0 元 规格：
25assays
产品信息
概述| 货号 | K110-25 |
| 描述 | Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (400 nm excitation filter and 505 nm emission filter)• Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/midd |
| 产品特色 | • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity. |
| 试剂盒组分 | • Cell Lysis Buffer • 2X Reaction Buffer • YVAD-AFC (1mM) • DTT (1 M) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
