Caspase-6 Fluorometric Assay Kit
货号：
K114-25 基本售价：
2118.0 元 规格：
25assays
产品信息
概述| 货号 | K114-25 |
| 描述 | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-6 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by Mch2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | fluorecence (400 nm excitation filter and 505 nm emission filter)• Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/middl |
| 产品特色 | • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity. |
| 试剂盒组分 | • Cell Lysis Buffer • 2X Reaction Buffer • VEID-AFC (1 mM) • DTT (1M) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
