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货号: bs-10634R 基本售价: 1380.0 元 规格: 100ul
- 规格:100ul
- 价格:1380.00元
- 规格:200ul
- 价格:2200.00元
产品信息
- 产品编号
- bs-10634R
- 英文名称
- HLA Class 1 ABC /HLA ABC
- 中文名称
- 人类白细胞抗原A抗体
- 别 名
- 1A01_HUMAN; HLA A; HLA B; HLA C; HLAabc; HLA class 1 A; HLA class 1 B; HLA class 1 C; Major histocompatibility complex, class I, A + B + C; MHC class I HLA A; MHC class I HLA B; MHC class I HLA C; MHC HLA ABC.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 细胞膜受体 细胞表面分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 38kDa
- 细胞定位
- 细胞浆 细胞膜 细胞外基质
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human HLA Class 1 ABC :151-250/365 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- Function:
Involved in the presentation of foreign antigens to the immune system.
Subunit:
Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Post-translational modifications:
Sulfated.
Polyubiquitinated in a post ER compartment through interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).
Similarity:
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
SWISS:
P30443
Gene ID:
3105
Database links:Entrez Gene: 3105Human
Omim: 142800Human
SwissProt: P04439Human
Unigene: 181244Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
- Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HLA ABC Polyclonal Antibody, Unconjugated(bs-10634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HLA ABC Polyclonal Antibody, Unconjugated(bs-10634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HLA ABC Polyclonal Antibody, Unconjugated(bs-10634R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei