| 货号 | 10006518-1ea |
| 描述 | Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology.1,2 NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive nitrogen species (RNS). Cayman’s S-Nitrosylated Protein Detection Kit (Biotin Switch) employs a modification of the Jaffrey et al. Biotin-switch method to allow for the direct visualization of S-NO proteins in whole cells or tissues, as well as by western blot analysis.3,4 Using this method, free SH groups are first blocked (an addition of 125.1 amu per residue) and any S-NO bonds present in the sample are then cleaved. Biotinylation of the newly formed SH groups (an addition of 523.6 amu per residue) provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. |
| 别名 | S-NO; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Alderton, W.K.,Cooper, C.E., and Knowles, R.G. Nitric oxide synthases: Structure, function, and inhibition. Biochemistry Journal 357, 593-615 (2001). 2.Bredt, D.S. Endogenous nitric oxide synthesis: Biological functions and pathophysiology. Free Radical Research 31, 577-596 (1999). 3.Ckless, K.,Reynaert, N.L.,Taatjes, D.J., et al. In situ detection and visualization of S-nitrosylated proteins following chemical derivatization: Identification of Ran GTPase as a target for S-nitrosylation. Nitric Oxide: Biology and Chemistry 11, 216-227 (2004). 4.Jaffrey, S.R.,Erdjument-Bromage, H.,Ferris, C.D., et al. Protein S-nitrosylation: A physiological signal for neuronal nitric oxide. Nature Cell Biology 3(2), 193-197 (2001). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |