| 货号 | 10004380-1ea |
| 描述 | Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of stimulated cells. The biological effects of PAF include activation of platelets, polymorphonuclear leukocytes, monocytes, and macrophages. PAF also increases vascular permeability, decreases cardiac output, induces hypotension, and stimulates uterine contraction.1 PAF has been implicated in pathological processes, such as inflammation and allergy.2 PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). PAF-AHs are located intra- and extra-cellularly (e.g., cytosolic and plasma). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.3 Recently, plasma PAF-AH has been linked to atherosclerosis and may be a positive risk factor for coronary heart disease in humans.4 The Cayman Chemical PAF Acetylhydrolase Inhibitor Screening Assay uses 2-thio PAF as a substrate for PAF-AH.5 Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5-dithio-bis-(2-nitrobenzoic acid) also known as DTNB or Ellmans reagent. Caymans PAF-AH Inhibitor Screening assay includes human plasma PAF-AH and is a time saving tool for screening vast numbers of inhibitors. |
| 别名 | Lp-PLA2;PAF-AH;Platelet-activating Factor Receptor Acetylhydrolase; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Prescott, S.M.,Zimmerman, G.A., and McIntyre, T.M. Platelet-activating factor. The Journal of Biological Chemisty 265, 17381-17384 (1990). 2.Snyder, F. Platelet-activating factor and related acetylated lipids as potent biologically active cellular mediators. American Journal of Physiology.Cell Physiology 259, C697-C708 (1990). 3.Stafforini, D.M.,Prescott, S.M., and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. The Journal of Biological Chemisty 262, 4223-4230 (1987). 4.Caslake, M.J., and Packard, C.J. Lipoprotein-associated phospholipase A2 (platelet-activating factor acetylhydrolase) and cardiovascular disease. Current Opinion in Lipidology 14, 347-352 (2003). 5.Aarsman, A.J.,Neys, F.W., and van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. European Journal of Biochemistry 200, 187-193 (1991). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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